Toxcicity of the Ethanolic Extract of Jimson Weed (Datura stramonium L.) on Albino Laboratory Mice (Mus musculus L.)

الملخص

Rats and mice belong to the suborder Myomorpha, family Muridae are the most feared pests.
They attack food crops and stored products, destroy or weaken man's constructions and cause
health problems. This study was designed to investigate aspects pertaining to Datura (Datura
stramonium (L.) seeds contents as a botanical rodenticide, aiming to open new horizons in the
area of chemical ecology by using Sudanese indigenous plants in the field of crop protection.
The experiments were carried out in Central Laboratory for Veterinary Research, Suba,
Khartoum, Sudan, in Season 2019 to assess the effect of the Ethanolic extract of datura seeds
on albino laboratory mice (3 males and 3 females for each dose). Using four doses (0.33,
1.00, 2.00 and 3.33) mg and distilled water for the control treatment, Complete Randomized
Design (CRD) was followed in data analysis and Duncan's Multiple Range Test (DMRT) was
used in the comparison of means. Toxicity evaluation was done through certain measurements
these were: calculating LD50, LD90 monitor toxicity symptoms and mice activity, weight
before and after treatment and blood test. Death occurred 2, 3, and 5 albino mice were
scarified after treatment corresponding to dosage levels of 1.00, 2.00 and 3.33 mg /mice
respectively. No death cases were recorded at 0.33mg dose and the control treatments. LD50
and LD90 values were calculated (LD50 (1.9 g/kg)) (LD90 (9.1 g/ kg) In general the results
revealed that significant differences between doses and the control treatment. Also the results
showed an increase in toxicity symptoms coinciding with increasing in the doses as well as
lack of motor activity. Weight loss has been observed in dose 1.00 mg/g, 2.00 mg/g, 3.33 mg/g and slightly decrease in dose 0.33 and control treatment . Also there were Changes in
the total blood count compared to the control treatment.